I. Microarray Related Links
1. Microarray Platforms
- Affymetrix GeneChips:
Affymetrix is a high-density
oligonucleotide GeneChip manufacturer. The Barley1 GeneChip is the first
Affymetrix high-density GeneChip for cereals. Affymetrix produces
GeneChips for a wide range of organisms, including barley, Arabidopsis, C.
elegans, yeast, human, rat, Drosophila, and E. coli.
- Spotted Microarrays:
Originally
used by Pat Brown's laboratory at Stanford University, when spotted arrays
consisted of cDNA representations of between 100 and 700 base pairs. Now, there
are also
custom oligonucleotide sets representing cDNAs developed and
available for spotting from manufacturers such as
Incyte,
Operon,
PGA Harvard and
MWG.
2. Microarray Data Analysis Tools
Used in BarleyBase
-
Bioconductor/R : Bioconductor
is an open source project aimed at providing comprehensive capability for
microarray data analysis, visualization and annotation. It started in fall
of 2001, and quickly evolved into a high-profile projects. It provides
packages for analyzing both Affymetrix oligo GeneChip data (affy, affydata
and affycomp packages) and cDNA spotted microarray.
BarleyBase extensively uses
R/Bioconductor in microarray data normalization, visualization and
online real-time analysis. BarleyBase used its Bioconductor's affy package for estimation of expression values by Robust Multi-chip
Average (RMA) and MAS5.0 methods. Dr. Dan Nettleton
explained how RMA normalization works.
Bioconductor is developed with
R, R is a
GNU project, an open
language and environment for statistical computing. It is a functional
programming language and has powerful graphical and visualization
capabilities.
-
MAS5.0: Affymetrix provides with
its Microarray Analysis Suite 5.0 the function to do absolute and
comparative expression estimation. MAS5.0 uses statistical algorithm for
normalization and calculate the expression values, the p-value and the
presence/absence call. BarleyBase uses the affy package of Bioconductor
for approximation of MAS5.0 methods in BarleyBase.
3. Microarray Databases
4. Microarray Database System
- BASE:
BioArray Software Environment (BASE) is a comprehensive
free web-based database solution for management and analysis
microarray analysis data.
-
GeneX-Lite-: Developed
at NCGR, GeneX-Lite is a client application that provides an interface to
a relational database management system (RDBMS). The client application
interface allows the user to load, manage, analyze, visualize and query
gene expression data.
5. Microarray Database Standard
-
MIAME:
Minimum information about a microarray experiment(
MIAME) is a
set of guidelines that can assist with the development of microarray
repositories and data analysis tools. It was Endorsed by
Microarray Gene Expression Data group (MGED.
Now it is accepted by major scientific journals including Nature and
Science and adapted by many microarray databases.
6.
Other Microarray Related Links:
The above-mentioned links are very limited in
range. So please trying some general link page to expand your reach:
-
SMD Microarray
Links : Software & tools, databases.
-
Gene Expression
Links
-
Listing of DNA microarray links
-
Plant
Arrays (Stanford)
-
National Cancer
Institute
II. Genome Databases and
Related Links
1. Barley Related Links
-
Barley Genome Dynamics (Schulman Group)
-
Barley genomics (Kleinhofs group)
-
cerealsDB.uk.net (Keith Edwards)
-
North American
Barley Genome Project
2. Other Plant Related Links
-
Arizona Genomics Institute
-
Cereal Genomics and Pathology (Wise
group)
-
Clemson University Genomics
Institute
-
Databases for
Molecular Biology
-
GrainGenes
-
Gramene
-
HarvEST
-
IPK Gatersleben
-
IACR - Long
Ashton
-
Max Planck Institute, Koln
-
NASC - Nottingham Arabidopsis Stock
Centre
-
National Plant Germplasm System
-
NCBI [National Center for
Biotechnology Information]
-
PlantGDB
-
Risø National Laboratory
-
Scottish Crop Research Institute
-
Sputnik
-
TAIR
-
TIGR, The Institute for Genomic Research
-
TREP -
Triticeae Repeat Sequence Database
-
UK Cropnet
III. Iowa State
University Links
-
Bioinformatics and Computational
Biology
-
Center for Plant Genomics
-
Center for Plant Responses
to Stress
-
Center for Plant
Transformation and Gene expression
-
Iowa State University Homepage
-
Laurence H. Baker Center
for Bioinformatics and Biological Statistics
-
Plant Pathology
-
Plant Sciences Institute
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