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Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions | |||||||||||
| Experiment Name: | Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions | ||||||||||
| Accession No. & GeneChip: | BB4, Barley1 | ||||||||||
| Submitter: | Rico A. Caldo | ||||||||||
| Experiment Type: | time course | ||||||||||
| Experiment Factor: | genotype pathogen isolates time | ||||||||||
| Number of Replicates: | 3 | ||||||||||
| Quality Control Steps: | biological replicates | ||||||||||
| Quality Control Description: | This study was conducted in three replications. | ||||||||||
| Publication_id: | PMID: 15319481
Rico A. Caldo, Dan Nettleton, and Roger P. Wise Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew Plant Cell 2004 16: 2514-2528. Reviewed by Nancy A. Eckardt in: http://www.plantcell.org/cgi/content/full/16/9/2249 Cutting Edge Transcriptome Analysis: It's All about Design | ||||||||||
| Last Update Time: | 2004-10-28 13:24:45 | ||||||||||
| Expression Data Access & Analysis: | Please select one action: 1. Batch download all data files at Download Center . 2. Browse Hybridizations and boxplots from experiment. 3. Visualization of hybridizations or treatment means with scatterplots and MvA plots. 4. Browse Samples from experiment. 5. Create Gene List by filter probe sets for differentially-expressed genes. 6. Pattern Recognition on filtered gene list. | ||||||||||
| Description: | A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13). | ||||||||||
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