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Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions

Experiment Name: Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions
Accession No. & GeneChip: BB4, Barley1
Submitter: Rico A. Caldo
Experiment Type: time course
Experiment Factor: genotype
pathogen isolates
Number of Replicates: 3
Quality Control Steps: biological replicates
Quality Control Description: This study was conducted in three replications.
Publication_id: PMID: 15319481 Rico A. Caldo, Dan Nettleton, and Roger P. Wise Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew Plant Cell 2004 16: 2514-2528.
Reviewed by Nancy A. Eckardt in:
Cutting Edge Transcriptome Analysis: It's All about Design
Last Update Time: 2004-10-28 13:24:45
Expression Data
Access & Analysis:

Please select one action:

1. Batch download all data files at Download Center .

2. Browse Hybridizations and boxplots from experiment.

3. Visualization of hybridizations or treatment means with scatterplots and MvA plots.

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5. Create Gene List by filter probe sets for differentially-expressed genes.

6. Pattern Recognition on filtered gene list.
Description: A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13).
Name: Rico Caldo
Institution: Iowa State University
Head of Laborotary: Roger P Wise


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